Briefly describe the basic principles of using absorption spectroscopy to determine the…

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1. Briefly describe the basic principles of using absorption spectroscopy to determine the concentration of a substance in a solution.

2. Why is a reference solution needed when determining the absorbance? Why is a blank solution usually used as the reference?

3. Each time after the wavelength is changed, transmittance “0” and “100%” need to be readjusted. Explain why. Please refer to the relationship between the light absorption of a substance and the wavelength.

 
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